mouse t lymphoma el4 cells  (ATCC)

Journal: ACS applied bio materials

Article Title: Dual Thermo- and pH-Responsive Polymer Nanoparticle Assemblies for Potential Stimuli-Controlled Drug Delivery.

doi: 10.1021/acsabm.4c01167

Figure Lengend Snippet: Figure 12. Cytostatic and cytotoxic effects of diblock polymers evaluated in mouse cancer cell lines and purified CD8+ T lymphocytes in vitro. Cytostatic effect of AB1, AB2, and AB3 determined in a) EL4, b) LL2 cell lines, and c) CD8+ T lymphocytes isolated from spleens of BALB/c mice using the [3H]-thymidine incorporation assay. Cytotoxic effect of AB1, AB2, and AB3 measured in d) EL4 and e) LL2 cell lines. Each experimental point represents the average value from four wells ± SD. Experiments were performed twice with similar results.

Article Snippet: The mouse T lymphoblastic lymphoma cell line EL4 was purchased from ATCC (Manassas, VA, USA) and cultured in RPMI-1640 medium (Sigma-Aldrich, Czech Republic) with the addition of heat-inactivated fetal bovine serum (10%), 100 U mL−1 of penicillin-streptomycin solution, 1 mM sodium pyruvate, 4.5 g L−1 of glucose, and 4 mM glutamine.

Techniques: Purification, In Vitro, Isolation, Thymidine Incorporation Assay

Journal: Frontiers in Immunology

Article Title: Cysteine-specific 89 Zr-labeled anti-CD25 IgG allows immuno-PET imaging of interleukin-2 receptor-α on T cell lymphomas

doi: 10.3389/fimmu.2022.1017132

Figure Lengend Snippet: 89 Zr-CD25 IgG uptake in mouse cells in vitro and in mouse lymphoma in vivo . (A) CD25 expression (left) and anti-mouse 89 Zr-CD25 IgG binding (right) in mouse thymus Treg lymphocytes and EL4 mouse lymphoma cells. Cell uptake was compared by using equivalent amounts of cells. Blocking was done by adding 0.5 μM of unlabeled anti-CD25 IgG. (B) Representative coronal (left) and transaxial (right) PET images at 5 days of EL4 tumor-bearing immunocompetent C57/Bl6 mice administered with anti-mouse 89 Zr-CD25 IgG alone (top) or co-injected and 0.8 mg of unlabeled anti-CD25 IgG for blocking (middle). Biodistribution data are shown (bottom) as the mean ± S.E of values obtained from two independent experiments (n = 6 per group) N.S., not significant.

Article Snippet: Human anaplastic large cell lymphoma SUDHL-1 cells and EL4 mouse T lymphoma cells were from the American Type Culture Collection and was tested negative for mycoplasma and authenticated by the Institutional Research Service.

Techniques: In Vitro, In Vivo, Expressing, Binding Assay, Blocking Assay, Injection